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ISOLATION OF CELLULOSE FROM SAND BOX (Hura crepitans) SEED SHELL
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Table of contents
CHAPTER ONE 1
1.1 Introduction 1
1.2 Aim and Objectives of the Study 2
CHAPTER TWO 3
2.0 Literature Review 3
2.1 Cellulose 3
2.1.1 Cellulose as a Polysaccharides 3
2.1.3 General structure of cellulose 5
2.1.4 Cellulose microfibril organization 8
2.1.5 Diffraction principle 10
2.2 Hura crepitans 11
CHAPTER THREE 13
3.0 Materials and Methods 13
3.1 Materials 13
3.1.1 Materials Sample Collection 13
3.1.2 Apparatus 13
3.1.3 Reagent 14
3.2 Methods 14
3.2.1 Isolation of Cellulose 14
3.2.2 Percentage Yield of Cellulose 15
3.2.3 Characteristics of Cellulose 15
3.3.3 Diffractometer technique 15
3.3.4 Sample preparation 16
CHAPTER FOUR 17
4.0 Results and Discussion 17
CHAPTER FIVE: Discussion and Conclusion 21
5.1 Summary 21
5.2 Conclusion 21
5.3 Recommendation 21
Appendix
References 22
Abstract
Cellulose is an important structural component of primary cell wall in green plant and about billion tons of cellulose is being produced from various plants. Cellulose is renewable, thermally stable, cheap, light in weight and the chemical formula (C6H10O5)n.
The cellulose was isolated by delignification process and alkaline hydrolysis. Percentage yield and proximate analysis were determined. It was characterized by FTIR, SEM and XRD.
The percentage cellulose yield was 10.14%. Fibre content of isolated Hura crepitans seed shell (1.68%) was observed to be lower than that for the isolated cellulose (3.11%). The moisture content of the isolated Hura crepitans seed shell (11.87%) was observed to higher than that of the raw Hura crepitans seed shell (11.79%). The percentage ash content (2.65%) of isolated Hura crepitans seed shell was also lower than that of raw (4.68%). Percentage crude fat (2.97) of isolates was lower than that of raw sample (2.18%). The broad band in the region 500-4500 cm-1 is due to O-H stretching vibration and it gives considerable information of the hydrogen bonds.
This study shows that Hura crepitans seed shell contains cellulose which is crystalline in nature.
Keywords: Hura crepitans, FTIR, SEM, Proximate Analysis, XRD, Cellulose
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