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ISOLATION OF BACTERIA FROM jURINARY TRACT INFECTION FROM STUDENTS OF OLABISI ONABANJO UNIVERSITY
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Table of contents
CHAPTER ONE: INTRODUCTION 1
1.1 Background to the Study 1
1.2 Statement of the Problem 3
1.3 Significance of the Study 4
1.4 Aim of the Study 4
1.5 Objectives of the Study 4
CHAPTER TWO: LITERATURE REVIEW 5
2.1 Urinary Tract Infections 5
2.2 Classification of UTI 8
2.2.1 Uncomplicated and complicated urinary tract infection 8
2.2.2 Recurrent urinary tract infection 9
2.3 Bacteria Associated with Urinary Tract Infections 10
2.3.1 Escherichia coli 10
2.3.2 Pseudomonas aeruginosa 11
2.3.3 Klebsiella pneumoniae 11
2.3.4 Proteus mirabilis 12
2.2.5 Enterococci 13
2.4 Routes of Transmission 13
2.3.1 Ascending route 14
2.3.2 Descending route 14
2.3.3 Lymphatic route 14
CHAPTER THREE: MATERIALS AND METHODS 16
3.1 Study Area 16
3.2 Collection of Samples 16
3.3 Materials and Media 16
3.4 Preparation of Culture Media 17
3.5 Isolation of Bacteria 17
3.6 Characterization and Identification of Bacteria Isolates 17
3.6.1 Gram Staining 17
3.6.2 Biochemical Characterization 18
CHAPTER FOUR: RESULTS 21
CHAPTER FIVE: DISCUSSION, CONCLUSION AND RECOMMENDATIONS 28
5.1 Discussion 28
5.2 Conclusion and Recommendations 29
References 30
LIST OF TABLES
TABLE TITLE PAGES
4.1 Physicochemical Characteristics of Urine Samples 23
4.2 Bacterial Load of Urine Samples 24
4.3 Morphological Characteristics of Isolate Recovered from Urine Samples 25
4.4 Morphological Characteristics of Isolate Recovered from Urine Samples 26
4.5 Antibiotic sensitivity pattern of bacterial agents isolated from urine samples 27
Abstract
The aim of this study was to isolate bacteria from urinary tract infected students of Olabisi Onabanjo University. Twenty-five (25) samples of urine were obtained from students during the period of June, 2019 to July, 2019. The participant age ranged between 18 to 26 years of age. Some of the urine samples were pale yellow, straw, and amber. The appearance of some of the sample were clear, cloudy and turbid. The pH of the samples ranged from 6.0 to 8.0. The microbial load of the urine samples ranged from 9.9 x 102 to 14.0 x104. The identified bacteria were Staphylococcus aureus (n= 18), Klebsiella pneumonia (n=13), Proteus spp. (n=7), Escherichia coli (n=3) and Pseudomonas aeruginosa (n=2). Antimicrobial susceptibility profile of the isolates showed that most of isolate were susceptible to Gentamycin (81.3%). The findings that majority of the isolates were showed resistance to CAZ, CEX, CTR, Erythromycin, CXC, OFL, and Augmentin. It implies that these antibiotics cannot be used as empirical therapy for urinary tract infection particularly in the study area.
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