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ISOLATION OF FUNGI FROM LECTURE THEATRES IN OLABISI ONABANJO UNIVERSITY,AGO IWOYE
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Table of contents
CHAPTER ONE 1
1.1 Introduction 1
1.2 Aims and Objectives 3
1.3 Objective of the study 3
1.4 Literature Review 10
CHAPTER TWO: Materials and Methods 10
2.0 study area 10
2.1 Material and method 10
2.1.1 Instrument and glass wares 10
2.1.2 Media Used 10
2.3 Method of Sterilization 11
2.2 sampling 11
2.3 culture method 12
2.3.1 Isolation and Identification of Fungal 12
2.3.2 Preparation of slide culture 13
2.3.3 Application of Stain 14
CHAPTER THREE: Results 15
CHAPTER FOUR: Discussion and Conclusion 22
4.2 Conclusion 23
References 25
LIST OF TABLES
List Title Page
3.1 Total Frequency of Airborne Fungi Isolated from Lecture Theartres 16
3.2 Mean Frequency and Percentage of Isolates 17
3.3 Morphology of Isolate Genera 18
LIST OF FIGURES
List Title Page
3.1 Primary fungal growth on Potatoes Detroxe Agar Medium (PDA)19
3.2 Isolated Fungi on PDA Media 20
3.3 microscopic pictures of Fungi Isolates 21
Abstract
Airborne fungi play an important role in causing allergy and infection in susceptible people. This study was conducted to isolate fungi in lecture theatre of Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria using the open plate method. Colonies grown on Potato dextrose agar were counted and identified by standard methods. A total of 35 colonies were Isolated. The genera of this isolated airborne fungi depending on frequency in number of colony counts were classified based on their dominance. The dominant species were Aspergillus niger (31.9%), Epidemophytin spp (17.0%), Cladosporium sp (29.8%), Penicillum spp. (21.4%). Aspergillus niger is the most prevalent with 42% colony count and Cladosporium spp less prevalent (8.6%). From the result obtained, there is need to safeguard the health of students, staffs and other workers by taking control measures that would inhibit the environmental factors that favors the growth of different fungi in the lecture theatres of the University.
Keywords: Airborne, Fungi, Colony count.
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