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ISOLATION OF FUNGI FROM LECTURE THEATRES IN OLABISI ONABANJO UNIVERSITY,AGO IWOYE

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Table of contents

CHAPTER ONE                                                                                                                              1

1.1     Introduction                                                                                    1

1.2     Aims and Objectives                                                                      3

1.3     Objective of the study                                                                    3

1.4     Literature Review                                                                          10

CHAPTER TWO: Materials and Methods                                              10

2.0     study area                                                                                       10

2.1     Material and method                                                                      10

2.1.1  Instrument and glass wares                                                            10

2.1.2 Media Used                                                                                    10

2.3     Method of Sterilization                                                                 11

2.2     sampling                                                                                         11

2.3     culture method                                                                               12

2.3.1  Isolation and Identification of Fungal                                            12

2.3.2  Preparation of slide culture                                                            13

2.3.3  Application of Stain                                                                       14

CHAPTER THREE: Results                                                                   15

CHAPTER FOUR: Discussion and Conclusion                                      22

4.2     Conclusion                                                                                    23

References                                                                                                25


LIST OF TABLES

List    Title                                                                                               Page

3.1     Total Frequency of Airborne Fungi Isolated from Lecture Theartres   16                                                          

3.2     Mean Frequency and Percentage of Isolates                                  17                                                                              

3.3     Morphology of Isolate Genera                                                       18                  


LIST OF FIGURES

List    Title                                                                                               Page

3.1     Primary fungal growth on Potatoes Detroxe Agar Medium (PDA)19                                                     

3.2     Isolated Fungi on PDA Media                                                      20

3.3     microscopic pictures of Fungi Isolates                                          21

Abstract

Airborne fungi play an important role in causing allergy and infection in susceptible people. This study was conducted to isolate fungi in lecture theatre of Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria using the open plate method. Colonies grown on Potato dextrose agar were counted and identified by standard methods. A total of 35 colonies were Isolated. The genera of this isolated airborne fungi depending on frequency in number of colony counts were classified based on their dominance. The dominant species were Aspergillus niger (31.9%), Epidemophytin spp (17.0%), Cladosporium sp (29.8%), Penicillum spp. (21.4%). Aspergillus niger is the most prevalent with 42% colony count and Cladosporium spp less prevalent (8.6%). From the result obtained, there is need to safeguard the health of students, staffs and other workers by taking control measures that would inhibit the environmental factors that favors the growth of different fungi in the lecture theatres of the University.

 

Keywords: Airborne, Fungi, Colony count.

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