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COMPARATIVE MICROBIAL ANALYSIS OF CEMENTED AND TILED BATHROOM OF STUDENT’S HOSTEL IN IJEBU-NORTH LOCAL GOVERNMENT AREA

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Table of contents

 

CHAPTER ONE                                                                                                                     1 

1.1       Introduction                                                                                                                1 

1.2       General Objective of the Study                                                                                  4 

1.3       Specific Objectives                                                                                                     4 

CHAPTER TWO: Literature Review                                                                                    5 

2.1       Microbial Flora of the Human Body                                                                          5 

2.2       Factors Involved in the Microbial Flora Infections                                                    6 

2.3       Bathroom and Sanitation                                                                                            7 

2.4       Potential Role of Bathroom as Vector for transmission of Infectious Diseases     8 

2.5       Effects of Poor Bathroom and Sanitation                                                                  10 

2.6       Potential Role of Bathrooms as Vector for Transmission of Infectious Disease       10 

 

CHAPTER THREE                                                                                                                15 

3.1       Study Area                                                                                                                  15 

3.2       Collection of Samples                                                                                                15 

3.3       Sterilization of Glassware                                                                                          15 

3.4       Isolation and Characterization of Organisms                                                             16 

3.5       Morphological Characterization                                                                                17 

3.5.1    Gram staining                                                                                                             17 

3.5.2    Motility Test                                                                                                               17 

3.5       Biochemical Tests                                                                                                      18 

3.5.1    Indole production test                                                                                                 18 

3.5.2    Coagulase test                                                                                                             18 

3.5.3    Catalase test                                                                                                                19 

3.5.4    Oxidase test                                                                                                                19 

3.5.5    Citrate utilization test                                                                                                 19 

3.5.6    Urease Test                                                                                                                 19 

CHAPTER FOUR: Results                                                                                                    20 

CHAPTER FIVE                                                                                                                    37 

5.1       Discussion                                                                                                                   37 

5.2       Conclusion and Recommendations                                                                            41 

References                                                                                                                              43 

LIST OF TABLES 

Table              Title                                                                                                           Pages 

4.1       Microbial counts recovered from cemented bathroom and tiled Bathroom                    21 

4.2       Morphology Characteristics of Suspected Organisms in the Cemented 

                        Bathroom                                                                                                        22

4.3       Morphology Characteristics of Suspected Organisms in the Tiled Bathroom          26 

4.4       Biochemical Characteristics of Isolated Organisms from Cemented Bathroom   30 

4.5       Biochemical Characteristics of Isolated Organisms from Tiled Bathroom                    32 

4.6       Frequency and Percentage of Organisms on Floor and Wall of Cemented 

                        and Tiled Bathroom                                                                                        35 

 

4.7       Frequency and percentage of organisms isolated from cemented and tiled 

Bathroom                                                                                                                    36 

Abstract

 

The circulation of infectious diseases in the community settings in urban and rural areas remains to be a hectic problem. One of the sources of microbial diseases is bathroom. The current study aim to compare the quality and quantity of microbes of tiled and cemented bathroom of student’s hostel in Ijebu-north Local Government Area as a public health issues. Moistened swab stick were used to collect samples from bathrooms in forty (40) cemented bathroom and forty (40) tiled bathrooms using sterile cotton swab stick moistened with sterile normal saline. The microbial and biochemical analysis were also determined using standard methods. The average mean for coliform count in tiled bathroom is 8.4 x 103 and it ranged from 1.2 x 103 to 9.9 x 103. The highest count was found in BF17 (Bathroom Floor) with 9.9 x 103, followed by BF5 (Bathroom Floor) with 8.6 x 103 and the least count was found in BF4 (Bathroom floor) and BW2 (Bathroom wall) with 1.2 x 103 (Table 4.1). The average mean for coliform count in cemented bathroom is 13.0 x 103 and it ranged from 1.6 x 103 to 13.2 x 103. The highest count was found in BF2 (Bathroom Floor) with 13.2 x 103, followed by BF20 (Bathroom Floor) with 12.1 x 103 and the least count was found in BF8 (Bathroom floor) with 1.6 x 103 (Table 4.1). A total of one hundred and thirty six were isolated from the samples collected (seventy-two isolates from cemented bathroom and sixty four isolates from tiled bathroom). The isolated organisms includes Escherichia coli, Proteus spp., Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis and Shigella dysentriae. It is recommended that both adult and young should adopt the habit of hand washing practice after using the bathrooms or  toilets and routine surface disinfection of the bathroom surface, this can prevent cross contamination. 

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