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COMPARATIVE MICROBIAL ANALYSIS OF CEMENTED AND TILED BATHROOM OF STUDENT’S HOSTEL IN IJEBU-NORTH LOCAL GOVERNMENT AREA
Categories
Table of contents
CHAPTER ONE 1
1.1 Introduction 1
1.2 General Objective of the Study 4
1.3 Specific Objectives 5
CHAPTER TWO 6
2.1 Literature Review 6
2.2 Organisms Associated with Restroom 8
2.3 Cleaning and Disinfection of Bathroom 10
2.4 Effect of Poor Bathroom and Toilet Sanitation on Human Health 12
2.5 Need for Improvement of Bathroom Sanitation and Hygiene Practice 14
CHAPTER THREE 15
3.1 Study Area 15
3.2 Collection of Samples 15
3.3 Sterilization of Glassware 15
3.4 Isolation and Characterization of Organisms 16
3.5 Morphological Characterization 17
3.5.1 Gram staining 17
3.5.2 Motility Test 17
3.5 Biochemical Tests 18
3.5.1 Indole production test 18
3.5.2 Coagulase test 18
3.5.3 Catalase test 19
3.5.4 Oxidase test 19
3.5.5 Citrate utilization test 19
3.5.6 Urease Test 19
CHAPTER FOUR: Results 20
CHAPTER FIVE 36
5.1 Discussion 36
5.2 Conclusion and Recommendations 38
References 40
LIST OF TABLE
Table Title Pages
4.1 Microbial counts recovered from cemented bathroom and tiled Bathroom 21
4.2 Morphology Characteristics of Suspected Organisms in the Cemented
Bathroom 22
4.3 Morphology Characteristics of Suspected Organisms in the Tiled Bathroom 26
4.4 Biochemical Characteristics of Isolated Organisms from Cemented Bathroom 30
4.5 Biochemical Characteristics of Isolated Organisms from Tiled Bathroom 32
4.6 Frequency and percentage of organisms isolated from cemented and tiled
Bathroom 36
Abstract
Sanitary conditions in public places such as bathrooms has always been a major concern to public health departments and they are continually checking the cleanliness and safety of the bacteria breeding places to prevent the spread of sickness and disease. In this study, the quality and quantity of microbes of tiled and cemented bathroom of student’s hostel in Ijebu-north Local Government Area as a public health issues was examined. Forty (40) samples were collected using sterile cotton swab stick moistened with sterile normal saline and analysed for bacteriological contamination. The coliform count in tiled bathroom ranged from 1.2 x 103 to 9.9 x 103. The coliform count in cemented bathroom ranged from 1.6 x 103 to 13.2 x 103. A total of one hundred and thirty six organisms were isolated from the samples collected out of which seventy-two isolates were from cemented bathroom while sixty four isolates were from tiled bathroom. The isolated organisms in cemented bathroom includes Escherichia coli, Proteus sp, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus sp and Shigella sp while isolated organisms from tiled bathroom includes Escherichia coli, Proteus sp, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Shigella sp. . It is therefore strongly recommended that public awareness and enlightenment campaigns on proper hygiene of bathroom and personal hygiene be intensified within the study area in particular and the general public.
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