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COMPARATIVE MICROBIAL ANALYSIS OF CEMENTED AND TILED BATHROOM OF STUDENT’S HOSTEL IN IJEBU-NORTH LOCAL GOVERNMENT AREA

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Table of contents

 

CHAPTER ONE                                                                                                                     1 

1.1       Introduction                                                                                                                1 

1.2       General Objective of the Study                                                                                  4 

1.3       Specific Objectives                                                                                                     5 

CHAPTER TWO                                                                                                                    6 

2.1       Literature Review                                                                                                       6 

2.2       Organisms Associated with Restroom                                                                       8 

2.3       Cleaning and Disinfection of Bathroom                                                                     10 

2.4       Effect of Poor Bathroom and Toilet Sanitation on Human Health                         12 

2.5       Need for Improvement of Bathroom Sanitation and Hygiene Practice                       14 

CHAPTER THREE                                                                                                                15 

3.1       Study Area                                                                                                                  15 

3.2       Collection of Samples                                                                                                15 

3.3       Sterilization of Glassware                                                                                          15 

3.4       Isolation and Characterization of Organisms                                                             16 

3.5       Morphological Characterization                                                                                17 

3.5.1    Gram staining                                                                                                             17 

3.5.2    Motility Test                                                                                                               17 

3.5       Biochemical Tests                                                                                                      18 

3.5.1    Indole production test                                                                                                 18 

3.5.2    Coagulase test                                                                                                             18 

3.5.3    Catalase test                                                                                                                19 

3.5.4    Oxidase test                                                                                                                19 

3.5.5    Citrate utilization test                                                                                                 19 

3.5.6    Urease Test                                                                                                                 19 

CHAPTER FOUR: Results                                                                                                    20 

CHAPTER FIVE                                                                                                                    36 

5.1       Discussion                                                                                                                   36 

5.2       Conclusion and Recommendations                                                                            38 

References                                                                                                                              40 


LIST OF TABLE

Table              Title                                                                                                           Pages 

4.1       Microbial counts recovered from cemented bathroom and tiled Bathroom                    21 

4.2       Morphology Characteristics of Suspected Organisms in the Cemented 

                        Bathroom                                                                                                        22 

 

4.3       Morphology Characteristics of Suspected Organisms in the Tiled Bathroom          26 

4.4       Biochemical Characteristics of Isolated Organisms from Cemented Bathroom   30 

4.5       Biochemical Characteristics of Isolated Organisms from Tiled Bathroom                    32 

4.6       Frequency and percentage of organisms isolated from cemented and tiled 

Bathroom                                                                                                                    36 

Abstract

 

Sanitary conditions in public places such as bathrooms has always been a major concern to public health departments and they are continually checking the cleanliness and safety of the bacteria breeding places to prevent the spread of sickness and disease. In this study, the quality and quantity of microbes of tiled and cemented bathroom of student’s hostel in Ijebu-north Local Government Area as a public health issues was examined. Forty (40) samples were collected using sterile cotton swab stick moistened with sterile normal saline and analysed for bacteriological contamination. The coliform count in tiled bathroom ranged from 1.2 x 103 to 9.9 x 103. The coliform count in cemented bathroom ranged from 1.6 x 103 to 13.2 x 103. A total of one hundred and thirty six organisms were isolated from the samples collected out of which seventy-two isolates were from cemented bathroom while sixty four isolates were from tiled bathroom. The isolated organisms in cemented bathroom includes Escherichia coli, Proteus sp, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus sp and Shigella sp while isolated organisms from tiled bathroom includes Escherichia coli, Proteus sp, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Shigella sp. . It is therefore strongly recommended that public awareness and enlightenment campaigns on proper hygiene of bathroom and personal hygiene be intensified within the study area in particular and the general public. 

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