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EVALUATION OF MICROBIAL PROFILE DURING FOOFOO PROCESSING
Categories
Table of contents
Title Page i
Abstract ii
Certification iii
Dedication iv
Acknowledgements v
Table of Contents vi-ix
List of Tables viii
List of Plate ix
Chapter One
Introduction 1-2
1.1 Background to the Study 2
1.2 Aim and Objectives 3
1.3 Justification
Chapter Two
Literature Review 4
2.0 Origin of Cassava 4-6
2.1 Processing of Cassava into different Food 6-9
2.2 Traditional Method of Foofoo Processing 10
2.3 Nutritional Changes 10-11
2.4 Microorganisms associated in Foofoo Processing 11-12
Chapter Three
Materials and Methods 13
3.0 Collection of Samples 13
3.1 Types Media 13
3.2 Preparation of Nutrient Agar 13
3.3 Preparation of MacConkey 14
3.4 Sabouraud Agar/ Potato Dextose Agar 14
3.5 Sterilisation of Apparatus 14
3.6 Microbiology Analysis 14-15
3.7 Apparatus and Equipment 15
3.8 Purification of Isolation 15-16
3.9 Sequence of operation of cassava foofoo production 17
3.10 Lactophenol Staining 17
Chapter Four
Results 18
4.1 Coding of samples obtained for testing 18
Chapter Five
Discussion and Conclusion 31
5.1 Discussion 31
5.2 Conclusion 32
References
LIST OF TABLES
Table 1. Processing steps in the production of some foofoo products in Africa 8
Table 4.1: Morphological and Biochemical Characteristics of bacteria Isolates recovered during the Stages involved in processing of Foofoo 21
Table 4.2: Morphological and Biochemical Characteristics of fungi Isolates recovered during the Stages involved in processing of Foofoo 27
Table: 4.3 Determination of pH Level 30
LIST OF PLATE
Plate 2.1: Flesh of the tuber 6
Abstract
Foofoo is a fermented wet-paste made from cassava. It is ranked next to garri as an indigenous food of most Nigerians in the South. There is need to determine the microbial content in the processing of foofoo so as to improve the production of foofoo. Three samples of peeled cassava, steep water, steep cassava, pulp mass and processed foofoo was collected from there different processors in Ijebu North Local Government in Ogun State. One sample was collected from Oru market, Mini Campus road and Olopomerin. Eight organisms were isolated namely: lactobaccillus spp., bacillus cereus, staphylococcus aureus, escherichia coli, penicillium spp. aspergillus niger, candida krusei , fusarium spp and rhizopus spp The range of bacteria count during the processing were 0.69 x 10-3 and 9.68 x10-3. For fungi: 0.01 x 10-3 and 0.48 x 10-3.
The presence of enteric bacteria in the processing of foofoo poses some potential risk to the consuming public, the need for improvement and maintenance of good hygienic practices by food handlers and vendors is emphasized to avert health hazard to the consumers.
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