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ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM AGRICULTURAL SOIL SAMPLES

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Table of contents

 

CHAPTER ONE                                                                                                                     1 

1.1       Introduction                                                                                                                1 

1.2       Statement of the Problem                                                                                           3 

1.3       Justification of Study                                                                                                  4 

1.4       Aim of the Study                                                                                                        4 

1.5       Objectives of the Study                                                                                              5 

CHAPTER TWO: Literature Review                                                                                    6 

2.1       General characteristics of actinomycetes                                                                   6 

2.2       Distribution of actinomycetes in natural habitats                                                       9 

2.2.1 Actinomycetes in plant rhizosphere soils                                                                      10 

2.3       Isolation of soil actinomycetes                                                                                   11 

2.3.1    Isolation media                                                                                                           11 

2.3.2    Physical or chemical pretreatment method                                                                12 

2.3.3    Other method for selective isolation of soil actinomycetes                                       12 

2.4       Identification and classification of actinomycetes                                                     13 

2.5       Actinomycetes as biological control agents                                                               15 

CHAPTER THREE: Materials and Methods                                                                         16 

3.1       Collection of Soil samples                                                                                          16 

3.2       Materials and Apparatus                                                                                             16 

3.3       Isolation of Actinomycetes                                                                                        16 

3.4.2    Biochemical Characterization                                                                                    17 

CHAPTER FOUR: Results                                                                                                    21 

CHAPTER FIVE                                                                                                                    26 

5.1       Discussion                                                                                                                   26 

5.2       Conclusion                                                                                                                  28 

References                                                                                                                              30 

 



 

LIST OF TABLES 

Table            Title                                                                                      Pages 

4.1      Total viable bacterial counts of soil samples                                                22 

4.2       Morphological Characteristics of Actinomycetes Recovered from the Soil 

Samples                                                                                                                      23 

4.3       Biochemical Reaction of Actinomycetes Recovered from the Soil Samples               24 

4.4       Frequency of occurrence of Actinomycetes Recovered from the Soil Samples         25 

Abstract

 

Actinomycetes are group of bacteria, which are present widely in ecological diversity. Actinomycetes are the major microbial population in soil which can produce active secondary metabolites. Aim of this study is to isolate and characterize varieties of actinomycetes from different agricultural soil. Soil samples were collected from different agricultural location (Vegetable soil, Maize soil, Cochorus soil, Banana soil, Cocoyam soil, Swampy soil, Stubborn grass soil and Forest soil) and used for actinomycetes isolation. The twenty-five actinomycetes classified into Streptomyces and Actinobacteria isolates were isolated from different soil samples based on their colony morphology. The isolates were analysed for their biochemical activity and found that all the isolates were positively active for catalase, motility and urease. 

Further study into the utilization of the actinomycetes for agriculture industry needs to be done to fully utilize these potential microbes for sustainable agriculture. 

 

Keyword: Agricultural soil, Actinomycetes, Streptomyces, Actinobacteria. 

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