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PREVALENCE AND ANTIBIOTICS RESISTANCE OF Salmonella serovars ISOLATED FROM STREET VENDED MEAT PIE
Categories
Table of contents
CHAPTER ONE: INTRODUCTION 1
1.1 Background to the Study 1
1.2 Aim of the Study 3
1.3 Objectives of the Study 4
1.4 Literature Review 4
CHAPTER TWO: MATERIALS AND METHODS 8
2.1 Collection of Sample 8
2.2 Material and Apparatus Used 8
2.3 Sterilization of Glasswares 9
2.4 Media Used 9
2.5 Media Preparation 9
2.6 Isolation of Salmonella isolates 9
2.7 Culture and Identification 10
2.8 Characterization of Salmonella 10
2.8.1 Microscopic Identification of Bacteria Isolates Gram Staining 11
2.8.2 Catalase Test 12
2.8.3 Oxidase Test 12
2.8.4 Citrate Utilisation Test 12
2.8.5 Urease Test 13
3.8.6 Indole Test 13
2.8.7 Kligler Iron Agar 13
2.9 Antimicrobial Susceptibility Testing 14
CHAPTER THREE: RESULT 16
CHAPTER FOUR: DISCUSSION, CONCLUSION
AND RECOMMENDATIONS 21
4.1 Discussion 21
4.2 Conclusion 23
4.3 Recommendations 24
References 25
Appendix 29
LIST OF TABLES
Table Title Page
3.1 Biochemical Characteristics of Salmonella Isolated from Street
Vended Meat Pie 19
3.2 Antimicrobial Resistance of Salmonella serovars isolates from
Street Vended Meat Pie Samples 20
LIST OF FIGURE
Figure Title Page
3.1 Morphological characteristics of Salmonella typhi and Salmonella
paratyphi on Salmonella-Shigella Agar 17
3.2 Antimicrobial sensitivity 18
Abstract
Salmonella serovars are one of the major causes of bacterial foodborne infection. The aim of this study was to determine the prevalence and antibiotic resistance of Salmonella serovars isolated from street vended meat pie. Out of the total 50 samples analysed from the meat pies, 29 meat pies proved to be positive to Salmonella typhi and Salmonella paratyphi. The Salmonella spp. isolated from the samples were tested against antibiotics which includes, ceftazidime, cefuroxime, gentamicin, ceftriaxone, erythromycin, cloxacilin, ofloxacin and augumentin. The most common resistance was observed in cefuroxime and ceftazidime while the most common susceptibility antibiotics are ceftriaxone and Ofloxacin. The study concluded that Salmonella isolates recovered from meat pies are commonly resistant to multiple antimicrobials. The study therefore recommends that relevant agencies such as Consumer protection Rights, NAFDAC and SON need to ensure and enforce strict compliance to Hazard Analysis Critical Control Points (HACCP) in all foods production sectors in Nigeria.
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