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PUBLIC HEALTH IMPLICATIONS OF MICROORGANISMS ISOLATED FROM SOME SWIMMING POOLS IN IJEBU-NORTH LOCAL GOVERNMENT
Categories
Table of contents
CHAPTER ONE 1
1.1 Introduction 1
1.2 Statement of the Problem 4
1.3 Significance of the Study 5
1.4 Objective of the Study 5
1.4.1 General Objective 5
1.4.2 Specific Objectives 5
CHAPTER TWO: Literature Review 6
2.1 Swimming Pool as Recreational Centre 6
2.2 Sanitary Conditions of Swimming Pool Water 7
2.3 Infectious diseases from Water Exposure 8
2.4 Transmission of Infectious Diseases via Swimming Pool Water 13
2.5 Commonly Used Microbial Indicators 14
CHAPTER THREE: Materials and Methods 17
3.1 Study Area 17
3.2 Collection of Samples 17
3.3 Material and Apparatus Used 17
3.4 Sterilization of Glasswares 18
3.5 Media Used and Preparation 18
3.6 Physical and Chemical Analysis of the Swimming Pool Water 18
3.7 Cultural Techniques 19
3.8 Identification of the Isolates 20
3.8.1 Morphological Characteristics 20
3.8.2 Biochemical Characteristics 21
3.8.1.1 Microscopic Identification of Bacteria Isolates Gram Staining 21
3.8.2.2 Catalase Test 22
3.8.2.3 Oxidase Test 22
3.8.2.4 Citrate Utilisation Test 22
3.8.2.5 Urease Test 23
3.8.2.6 Indole Test 23
3.8.2.7 Methyl Red Test 23
CHAPTER FOUR: Results 25
CHAPTER FIVE 33
5.1 Discussion 33
5.2 Conclusion 36
5.3 Recommendation 37
References 38
TABLE OF CONTENTS
Table Title Pages
4.1 Physiochemical Parameters of Swimming Pool Water in Ijebu-North Local
Government Area 26
4.2 The total microbial load count of isolates from the various swimming pool
water in Ijebu-North Local Government Area 27
4.3 Morphological Characterization of Bacteria Isolated 28
4.4 Biochemical Reaction of Bacterial Organisms Isolated from Swimming
Pool Water in Ijebu-North Local Government Area 31
Abstract
Water is essential for life and in most parts of the world including Nigeria, it is used for various activities such as drinking, bathing and recreational purposes. The aim of this study was to investigate public health implications of microorganisms isolated from some swimming pools in Ijebu-north local government area in Ogun State. Swimming pool water samples were collected from five different hotels in Ijebu-North, Ogun state, Nigeria. Physical and chemical parameters such pH, temperature, turbidity, odour and colour were carried out. The microbial and biochemical analysis were also determined using standard methods. The result of physical and chemical analysis shows that the water samples were colourless, odourless and some were blue amber while the temperature ranged from 25.2oC to 27.5oC, the pH ranged from 6.01 to 7.41. The microbial count ranged from 1.01 x 10-4 to 3.21 x 10-4 cfu/100ml. The result of the bacteria isolates revealed that five bacteria genera were isolated, these includes Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Enterobacter spp. and Klebsiella pneumonae. From these results, it can be concluded that most of the bacteriological and physiochemical parameters measured from swimming pools were not in compliance with the reference value set out by WHO guideline. This is mainly due to lack of awareness, absence of monitoring, proper disinfectant and lack of information or control over related parameters to efficient disinfection process. It is suggested that the observed problems can be minimized by promoting good hygiene education and practices for swimmers, applying a better and strict supervision, providing training and education for operators.
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