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BACTERIA ISOLATED FROM ROOT OF NEEM TREE AND THEIR ANTIBACTERIAL ACTIVITY
Categories
Table of contents
CHAPTER ONE 1
1.1 Introduction 1
1.2 Aim and Objectives of the Study 3
1.2.1 Aim 3
1.2.2 Objectives 3
1.3 Significance of the Study 4
CHAPTER TWO: Literature Review 5
2.1 Overview of Neem Tree 5
2.2 Review of Related Studies 7
CHAPTER THREE: Material and Methods 11
3.1 Study Site 11
3.2 Collection of samples 11
3.3 Sterilization of materials 11
3.4 Preparation of the sample 11
3.5 Isolation of Microorganisms 12
3.5.1 Media preparation 12
3.5.2 Inoculation of sample 12
3.5.3 Sub Culturing of Microoganisms 12
3.6 Morphological and Biochemical Characterization of bacteria 13
3.6.1 Morphological Characterization 13
3.6.2 Biochemical Characteristics 14
3.7 Test Microorganisms for Antibacterial Activity Determination 16
3.8 Test for Antibacterial activity 16
CHAPTER FOUR 17
4.0 Results 17
CHAPTER FIVE 24
5.0 Discussion, Conclusion and Recommendations 24
5.1 Discussion 24
5.2 Conclusion 25
5.3 Recommendations 26
References 27
Appendices 30
LIST OF TABLES
TABLE TITLE PAGES
4.1 Total Bacteria Count from Neem Root 19
4.2 Cultural Characteristics of Bacteria Isolates 20
4. 3 Gram Reaction and Biochemical Characteristics of Isolates 21
4.4 Zone of Inhibition of Bacillus spp. against Test Organisms 22
4.5 Zone of Inhibition of Streptomyces spp. against Test Organisms 23
Abstract
The aim of this study was to isolate bacteria from root of neem tree and determine their antibacterial activity. Neem root samples were collected from different location in Ago iwoye. One (1) g of the root sample was soaked in 10ml peptone water to dislodge the bacterial and also encourage growth and serial dilution was carried out after which where dilution factor 10-2 and 10-4 were used to culture the bacterial using pour plate method. The total bacteria count from neem root samples ranged from 1.0 x 102 to 6.8 x 102. A total number of twenty one (21) isolates were identified from all the root samples. The identified bacteria were Pseudomonas, Staphylococcus, Bacillus and Streptomyces. The antibiotic producing bacteria that were tested against pathogenic organisms were Bacillus spp. and Streptomyces. The isolated bacteria were tested against Staphylococcus aureus, Escherichia coli, Streptococcus fecalis, Pseudomonas aeurugenosa, Klebsiella pneumoniae and Salmonella typhi. Results from this study revealed the presence of antibacterial producing bacteria isolated neem roots. It was revealed that Bacillus spp. and Streptomyces spp. isolated from root of neem tree inhibited some of the test organisms which included Staphylococcus aureus, Escherichia coli, Streptococcus feacalis, Pseudomonas aeruginosa Klebsiella pneumoniae and Salmonella typhi. The results indicated that bacteria isolated from neem tree root had beneficial effect in controlling the pathogenic microbial organisms and thus can be used in therapeutic formulations in near future.
Keywords: Neem root, Antibacterial activity, Pathogenic organisms.
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