CHAPTER ONE

1.1 Introduction                                                     1

1.2 Aim of project                                                  3

CHAPTER TWO

Literature review                                                    4

MATERIAL AND METHODS

2.1   Sample collection                                            17

2.2.1  Nutrient agar                                                18

2.2.2  Macconkey agar                                            18

2.3.1   Isolation of total hetetrophic bacteria count     19

2.4   Identification of bacteria isolates                       20

2.5  Gram reaction                                                 20

2.6  Biochemical characterization of the isolates          21

2.6.2  Oxidase test                                                 21

2.6.3  Indole test                                                    22

2.6.4  Motility test                                                  23

2.6.5   Citrate test                                                  23

2.6.6  Spore stain test                                            24

2.6.7  Mannitol fermentation (Sugar)                        25

2.6.8  Coagulase                                                    25

2.6.9  Lactose fermentation                                     26

2.6.10  Urease test                                                 26

2.7.1  Pathogenicity test                                          27

2.7.1.1  Medium preparation                                    27

2.7.1.2  Method of culture                                       28

2.7.2  Antimirobial susceptibility testing                            28

2.7.2.1  Prparation of antibaceterial disc                    29 

CHAPTER THREE

RESULT

3.1 Count of distinct colonies of the isolates                      30

3.1.1  Microbial load of contaminated soil sample               30

3.1.2  Bacterial count for sample B                                  31

3.1.3  Microbial load for sample C (contaminated 

          Bathroom)                                                   31

3.1.4  Microbial load for sample D (cleaned bathroom  32

3.3   Microbiological analysis                                    33

3.3.1  Result of Pathogenicity Tests                           33

3.3.2  Result of Antimicrobial susceptibility                 35

CHAPTER FOUR

DISCUSSION, CONCLUSION AND RECOMMENDATION          38

4.1 Discussion                                                        38

4.2 Conclusion                                                       43

4.3 Recommendation                                              44

       References                                                      45

The bacteriological status of soil environment polluted with urine was analyzed, using standard microbiological methods. 

The identification test of bacteria revealed the isolation of proteus spp. Pseudmonas spp, Escherichia coli, Enterobacteria and Staphylococcus aureus from the contaminated soil. Bacillus spp, Pseudomonas spp and Staphylococcus aureus were obtained from the polluted bathroom and klebsiella spp, Pseudomonas spp, and Escherichia coli were isolated from the cleaned bathroom. Pathogenicity test carried out revealed that while all isolates from the polluted bathroom except E. coli to be pathogenic, only proteus from contaminated soil was found to be pathogenic amongst the isolates from contaminated and uncontaminated soil. Susceptibility tests from contaminated and uncontaminated soil susceptibility test revealed that among the disinfectants used for the study namely, Detol, Izal, Lysol and jik, Lysol was found to be effective against all the test pathogens. The study showed the diversity of pathogens in polluted environment and the efficacy of Lysol in decontaminating the polluted environment that harbour pathogenic organisms, but can be controlled through disinfection.