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FUNGAL PATHOGENS ASSOCIATED WITH POST-HARVEST ROT OF Capsicum annum (TATASE) AND Capsicum chinense (ATARODO)

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Table of contents

CHAPTER ONE                                                                                                                    1

1.1       Introduction                                                                                                                1

1.2       Objectives of the Study                                                                                              3

CHAPTER TWO: LITERATURE REVIEW                                                                                    4

2.1       Classification and Taxonomy of Pepper                                                                     4

2.2       Morphology and Growth of Pepper                                                                           5

2.3       Importance of Pepper                                                                                                 5

2.4       Pepper Breeding                                                                                                         7

2.5       Caspsicum anunum (Red Bell Pepper)                                                                       8

2.5.1    Importance and uses                                                                                                   9

2.6       Capisicum chinense (Ata Rodo)                                                                                 10

2.6.1    Importance of Capisicum chinense                                                                             10

2.7       Growth Requirement of Pepper                                                                                  10

2.7.1        Soil                                                                                                                              11

2.7.2        Rainfall and Temperature                                                                                           11

2.7.3    Fertilizer Application                                                                                                  11

2.7.4    Flowering and Fruit Set                                                                                              12

2.7.5    Harvesting                                                                                                                   12

2.7.6    Pests and Diseases                                                                                                      12

2.8       Factors Affecting Quality and Shelf- Life of Modified Atmosphere Products         13

2.8.1    Gaseous environment                                                                                                  13

2.8.2    Packaging material                                                                                                      14

2.9       Types of Packaging Materials Used In Modified Atmosphere Storage of Pepper`   14

2.9.1    Wood                                                                                                                          14

2.9.2    Plastic                                                                                                                          15

2.9.3    Jute sack bag                                                                                                               15

2.9.4    Glass                                                                                                                           16

CHAPTER THREE: MATERIALS AND METHODS                                                        17

3.1       Sample Collection                                                                                                       17

3.2       Sample Preparation                                                                                                     17

3.3       Glassware and Equipment Used                                                                                 17

3.4       Sterilization of Glassware                                                                                           17

3.5       Preparation of Potato Dextrose Agar                                                                         18

3.6       Preparation of Malt Agar                                                                                            18

3.7       Preparation of Sabouraud Dextrose Agar                                                                   18

3.8       Isolation of Fungi                                                                                                       18

3.9       Identification of Fungi                                                                                               18

CHAPTER FOUR: RESULTS                                                                                              18

4.1       Temperature of Capsicum chinense (Rodo) and Capsicum annum (Tatase) 

Stored under Different Condition                                                                              20

4.2       Growth of Fungi on Potato Dextose Agar, Malt Agar and Sabouraud Dextrose Agar 22

4.3       Fungi Load on Stored in Capsicum chinense (Rodo) and Capsicum annum (Tatase)  24

4.4       Morphological Characteristics of Fungal on SDA                                                      26

4.5       Morphological Characteristic of Fungal on PDA                                                       26

4.6       Morphological Characteristics of Fungal on Malt Agar                                             36

4.7       Identification of Fungal Isolated from Capsicum chinense (Rodo) and 

Capsicum annum (Tatase)                                                                                          30

CHAPTER FIVE: DISCUSSION AND CONCLUSION                                                   35

5.1       Discussion                                                                                                                   35

5.2       Conclusion                                                                                                                  36

References                                                                                                                              37



 

LIST OF TABLES

TABLE          TITLE                                                                                                PAGES

4.1       Temperature of the storage of Capsicum chinense (Rodo) and 

Capsicum annum (Tatase)                                                                                          21

4.2       Growth on PDA, Malt and SDA                                                                                23

4.3       Fungal Load on Stored in Capsicum chinense (Rodo) and 

Capsicum annum (Tatase)                                                                                          25

4.4       Morphological Characteristic of Fungal on in SDA                                                   27

4.5       Morphological Characteristic of Fungal on PDA                                                       28

4.6       Morphological Characteristics of Fungal on Malt Agar                                             29

4.7       Identification of Fungal Isolated from Capsicum chinense (Rodo) 

            and Capsicum annum (Tatase)                                                                                   31

Abstract

This study investigated the effect of post-harvest storage system on Capsicum annum (tatase) and Capsicum chinense (atarodo). Capsicum annum and Capsicum chinense were bought from the local market in Ago-Iwoye, Ogun State. Also, a bunch of Thamococcus daneilii leaves were also purchased with matted baskets. The first basket was laid with the leaves of T. daneilii before 15g of pepper were kept there while the second basket was not laid with the T. daneilli leaves. Each storage system was observed until spoilage occurred while temperatures were observed everyday for each sample. Fungi were isolated on Potato dextrose Agar, Malt Agar and Sabouraud Dextrose Agar. The temperature for Capsicum chinense laid with Thomatococcus danielli in a basket ranged from 30oC to 42oC. The highest temperature was observed in day 6 while the least temperature was observed in day 2. The temperature for stored Capsicum chinense in open basket ranged from 30oC to 45oC. The highest temperature was observed in day 6 while the leaset temperature was observed in day 2. The temperature for Capsicum annum stored in open basket ranged from 30oC to 38oC. The highest temperature was observed in day 6 while the least temperature was observed in day 2 and 3. The temperature for Capsicum annum laid with Thomatococcus danielli in a basket ranged from 29oC to 33oC. The highest temperature was observed in day 6 while the least temperature was observed in day 3. Results obtained showed that fungi load of Rodo (Capsicum chinense) in an open basket revealed 54.18% fungi load from the experiment. The next fungi laid with Thomatococcus danielli of Capsicum annum in a woven basket revealed 9.62% fungi load from the experiment and being the third highest. The next fungi load of Capsicum annum in an open basket revealed 7.7% fungi load from the experiment and being the lowest. And the last fungi load is laid with Thomatococcus danielli of Capsicum chinense in a basket revealed 27.9% fungi load from the experiment and being the second highest, and the total of fungi load is 52. It was concluded that packaging of Capisicum annuum and Capisicum chinense in basket with laid Thomatococcus danielii seems to hold promise in improving the shelf-life and maintaining the quality, as against storing in open woven basket. 

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