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MICROBIAL PROFILE OF SUYA MEAT IN ENUGU STATE
Categories
Table of contents
CHAPTER ONE- - - - - - - - -1
1.0 Introduction- - - - - - - - -1 1.1 Back ground of study- - - - - - -1
1.2 Aim And Objectives - - - - - - -4 CHAPTER TWO- - - - - - - -5
2.0 Literature review- - - - - - - -5 2.1Suya meat- - - - - - - - -5
2.2 Preparation of suya- - - - - - - -5
2.3 Microbiology of meat- - - - - - -6 -
2.4 Meat spoilage- - - - - - - - -7
2.5 Factors that affect the growth of microorganisms in meat- -8
2.5.1 Temperature- - - - - - - - -8
2.5.2 pH- - - - - - - - - -9
2.5.3. Water availability - - - - - - - -9
2.5.4 Nutrients- - - - - - - - -10
CHAPTER THREE- - - - - - - -11
3.0 Materials and methods- - - - - - -11
3.1 List of reagents- - - - - - - -11
3.2 List of glassware- - - - - - - -13
3.3 Preparation of media- - - - - - - -14
3.4 Sample collection - - - - - - - -14
3.5 Pretreatment of samples- - - - - - -15
3.6 Determination of total viable counts- - - - -15
3.6.1 Gram reaction- - - - - - - -16
3.6.2 Motility test- - - - - - - - -17
3.6.3 Catalase test - - - - - - - -17
3.6.4 Coagulase test - - - - - - - -18
3.6.5 Oxidase test- - - - - - - - -18
3.6.6 Urease test- - - - - - - - -19
3.6.7 Citrate test - - - - - - - - -19
3.6.8 Vogues Proskauer test - - - - - - -20
3.6.9 Indole test- - - - - - - - -21
3.6.10 Carbohydrate fermentation test- - - - -21
3.6.11 Methyl red test- - - - - - - -22
CHAPTER FOUR- - - - - - - -23
4.0 Results - - - - - - - - - -23
CHAPTER FIVE- - - - - - - -29
5.1 Discussion- - - - - - - - -29
5.2 Summary and Conclusion- - - - - - -31
5.3 Recommendations - - - - - - - -32
References- - - - - - - - -33
Appendix- - - - - - - - -37
LIST OF TABLES
TABLE 1: Total viable and coliform counts - - - - -25
TABLE 2: Characterization /identification of Isolate- - - -26
TABLE 3: Frequency of occurrence of isolates- - - - - 28
Abstract
Twenty (20) samples of suya meat in Enugu were collected randomly and analyzed microbiologically and the isolates were identified as Staphylococcus aureu(35%), Escherichia coli (15%), Streptococcus species (15%), Pseudomonas (35%). The most frequently isolated organisms were Staphylococcus and Pseudomonas. The total viable bacterial counts ranged from 1.9x 3.8x cfu/g whereas, total coliform count ranged from 1.1x-3.0x cfu/g on Nutrient agar and MacConkey agar respectively. The result revealed that the hygienic condition of the meat was below acceptable standard for human comsumption.
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