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MICROBIOLOGICAL ANALYSIS ON THREE RETAIL CORN-BASED SNACKS FROM AGO-IWOYE AND IJEBU-ODE MARKETS (TAFIRI, MASA AND AADUN)
Categories
Table of contents
CHAPTER ONE: Introduction 1
1.1 Background to the Study 1
1.2 Aim of the Study 4
1.3 Objectives of the Study 4
1.4 Justification of the Study 4
CHAPTER TWO: Literature Review 6
2.1 Maize: Production and Classification 6
2.2 Origin of maize 6
2.3 Corn Snacks 8
2.3.1 Donkwa 9
2.3.2 Masa 10
2.3.2 Aadun 11
2.4 Microbial Quality of Corn Powder Snacks 12
CHAPTER THREE: Materials and Methods 15
3.1 Study Area 15
3.2 Sample Collection 15
3.3 Media 15
3.4 Chemicals 15
3.5 Equipments 15
3.6 Sterilization of Materials 16
3.7 Preparation of Culture Media 16
3.7.1 Preparation of Nutrient Agar (NA) 16
3.7.2 Preparation of Eosine Methylene Blue Agar (MA) 16
3.7.3 Preparation of Potato Dextrose Agar (PDA) 17
3.8 Isolation of Microorganisms 17
3.9 Characterization and Identification of Biochemical Characteristics 17
3.9.1 Gram Staining Techniques 17
3.9.2 Lactophenol staining 18
3.9.3 Biochemical Tests 18
3.9.4 Sensory evaluation 20
3.10 Determination of pH 21
3.11 Determination of Moisture Content 21
CHAPTER FOUR: Results 22
4.1 Results 22
CHAPTER FIVE: Discussion, Conclusion and Recommendations 27
5.1 Discussion 27
5.2 Conclusion and Recommendations 27
References 30
LIST OF TABLES
TABLE NUMBER TITLE PAGE
4.1 Microbial counts recovered from retail corn snacks samples 22
4.2 Morphological and Biochemical Characteristics of Isolates Recovered
from Retail Corn Snacks 23
4.3 Morphological characterization of fungal isolates 24
4.4 Organosensory of retail corn snacks immediately after Production 25
4.5 Organosensory of retail corn snacks after storage 26
Abstract
Retailed corn snacks are usually consumed by lot of people. The study microbiologically analysed three retailed corn based snacks in Ago-Iwoye and Ijebu-Ode. A total number of thirty six (36) corn based snacks comprising Aadun (12), Tafiri (12) and Masa (12) were collected from vendors in Ago-Iwoye and Ijebu-Igbo in Ogun State, immediately after production and after storage. The samples were analyzed for bacteria and fungi using standard microbiological procedures. Immediately after production, the pH of the corn snack samples immediately after production ranged from 6.2 to 6.5; the moisture content ranged from 1.77±0.12 to 3.88±0.00. After storage, the pH ranged from 5.0 to 6.1; the moisture content ranged from 2.48±0.23 to 4.78±0.00. Analysis of the corn snacks samples revealed total bacterial viable count of the retail corn snacks immediately after production ranged from 0.1 x 102 to 11.8 x 102 Cfu/g while that of after storage ranged from 0.1 x 102 to 12.1 x 102 Cfu/g. The coliform count of the retail corn snacks immediately after production ranged from 3 to 11 MPN/g while that of after storage ranged from 4 to 39 MPN/g. There was no fungi growth on all the retail corn snacks immediately after production but there was fungi growth after storage, and the total fungal viable count after storage ranged from 0.1 x 101 to 0.5 x 101. The bacteria recovered from the samples include, Staphylococcus aureus, Salmonella spp. Pseudomonas spp., Shigella spp. and Escherichia coli. The isolated fungi were Aspergillus flavus, Mucor sp., Aspergillus niger and Aspergillus fumigatus. The organosensory quality of retail corn snacks immediately after production was also higher than after storage. The presence of these bacterial and fungal species could create health hazards when they come in contact with food during preparation and production. Therefor those involved in the processing of retail corn snacks should be aware of possible contamination and must adhere to strict sanitary measures in order to curb contamination.
Keywords: Corn, Snacks, Aadun, Tafiri, Masa
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