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MYCOFLORA AND PROXIMATE ANALYSIS OF COOKIES SOLD IN AGO- IWOYE, OGUN STATE.
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Table of contents
CHAPTER ONE: Introduction 1
1.1 Introduction 1
1.2 Aim and Objectives of the Study 4
1.2.1 Aim 4
1.2.2 Objectives of the Study 4
CHAPTER TWO: Literature Review 5
2.1 Cookies 5
2.2 Cookies Production 9
2.3 Wheat as Main Ingredients of Cookies 11
2.4 Microbial Quality of Cookies 13
2.5 Proximate Analysis of Cookies 15
2.6 Packaging and Storage of Cookies 27
CHAPTER THREE 31
3.0 Materials and Methods 31
3.1 Study Area 31
3.2 Collection of samples 31
3.3 Equipment and Materials Used 31
3.4 Sterilization of Glasswares 31
3.5 Media Preparation 32
3.6 Enumeration of Yeast and Mould 32
3.7 Characterization and Identification of Isolates 32
3.8 Proximate Analysis of the Samples 33
3.8.1 Determination of Crude Protein 33
3.8.2 Determination of Crude Fat 33
3.8.3 Determination of Ash Content 34
3.8.4 Determination of Moisture Content 34
3.8.5 Determination of Carbohydrate 35
3.8.6 Determination of Crude Fiber 35
CHAPTER FOUR: Results 36
CHAPTER FIVE: Discussion, Conclusion and Recommendations 40
5.1 Discussion and Conclusion 40
5.2 Recommendations 42
References 44
LIST OF TABLES
TABLE TITLE PAGES
4.1 Proximate Analysis of Cookies Samples 37
4.2 Macroscopic Identification of Isolated Fungi 39
Abstract
This study investigated the mycoflora associated with cookies on sale in Ago-Iwoye, Ogun State using standard microbiological methods. The proximate composition of the cookies were also estimated using standard biochemical methods. Mycoflora associated with the cookies includes; Aspergillus and Fusarium spp. Proximate analysis shows that the moisture content ranged from 1.66±0.08 to 5.37±0.01, crude fat ranged from 10.67±16.45±0.35, ash content ranged from 0.96±0.04 to 2.45±0.25, crude protein ranged from 5.49±0.21 to 6.85±0.03, crude fiber ranged from 1.32±0.04 to 3.20±0.01, while the carbohydrate ranged from 73.77±0.01 to 80.68±0.18. Adequate post production, handling and storage of products, especially during sale is important to reduce contamination of products.
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