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MYCOLOGICAL ENUMERATION OF FILES STORED IN MICROBIOLOGY DEPARTMENT
Categories
Table of contents
CHAPTER ONE: Introduction 1
1.1 Background to the Study 1
1.2 Statement of the Problem 4
1.2 Aim and Objectives of the Study 4
1.2.1 Aim 4
1.2.2 Objectives 4
CHAPTER TWO: Literature Review 5
2.1 Sources and types of microorganisms in archives and libraries 7
2.2 Biological destruction of paper 7
2.3 Health risks resulting from the presence of microorganisms in archives and libraries 10
2.4 Prevention against Biodeterioration of Books in Archives and Libraries 13
CHAPTER THREE: Materials and Methods 16
3.1 Study Area 16
3.2 Materials 16
3.2 Collection of samples 16
3.3 Media Preparation 17
3.4 Isolation of the Microorganisms from the stored files samples. 17
3.5 Morphological Characterization 17
3.6 Biochemical Test 18
3.6.1 Catalase Test 18
3.7 Identification of fungi 18
3.8 Antibiotics sensitivity test 18
CHAPTER FOUR: Results 20
CHAPTER FIVE: Discussion, Conclusion and Recommendation 28
5.1 Discussion 28
5.2 Conclusion 29
5.3 Recommendation 29
References 30
LIST OF TABLES
TABLE TITLE PAGES
4.1 Total Viable Count for Bacteria 22
4.2 Microscopic and morphological characteristics fungal Isolated from stored files 23
4.4 Percentage occurrence of fungal isolated from stored Files 24
4.5 Total Viable Count Fungi 25
4.6 Microscopy and Morphology Characteristics of Bacteria Isolated From Stored Files26
4.7 Zone of Inhibition of Isolated Organisms to Common Antibiotics 27
Abstract
The mycological enumeration of stored files in the microbiology department was studied and carried out in the microbiology laboratory of Olabisi Onabanjo University. Bacteria were isolated by pour plate method on nutrient agar. Several tests including biochemical test like catalase test were carried out on the isolate from the stored files. The bacteria identified were Streptococci and Diplococci. The fungi identified were Aspergillus sp., Candida spp., Mould spp., Rhizopus spp., and Scopolariopsis spp. The percentage occurrence of the fungal isolates showed that, Aspergillus spp. were more predominant with the highest percentage with 32% and Candida spp. has the least percentage occurrence with 5%. Both Mould spp. and Rhizopus spp. had 23% each and Scopolariopsis spp. had 17%. These results underscore the importance of moderating human activities to limit biodeterioration of printed materials. Printed materials should be shielded away from direct sunlight or other UV-light sources that may encourage paper degradation.
Keywords: Stored Files, Bacteria, Fungi, biodeterioration.
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