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MYCOLOGICAL ENUMERATION OF FILES STORED IN MICROBIOLOGY DEPARTMENT

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Table of contents

CHAPTER ONE: Introduction                                                                                              1

1.1       Background to the Study                                                                                           1

1.2       Statement of the Problem                                                                                           4

1.2       Aim and Objectives of the Study                                                                               4

1.2.1    Aim                                                                                                                             4

1.2.2    Objectives                                                                                                                   4

CHAPTER TWO: Literature Review                                                                                     5

2.1       Sources and types of microorganisms in archives and libraries                                  7

2.2       Biological destruction of paper                                                                                   7

2.3       Health risks resulting from the presence of microorganisms in archives and libraries 10

2.4       Prevention against Biodeterioration of Books in Archives and Libraries                  13

CHAPTER THREE: Materials and Methods                                                                         16

3.1       Study Area                                                                                                                  16

3.2       Materials                                                                                                                     16

3.2       Collection of samples                                                                                                  16

3.3       Media Preparation                                                                                                       17

3.4       Isolation of the Microorganisms from the stored files samples.                                 17

3.5       Morphological Characterization                                                                                  17

3.6       Biochemical Test                                                                                                         18

3.6.1    Catalase Test                                                                                                               18

3.7       Identification of fungi                                                                                                            18

3.8       Antibiotics sensitivity test                                                                                          18

CHAPTER FOUR: Results                                                                                                    20

CHAPTER FIVE: Discussion, Conclusion and Recommendation                                        28

5.1       Discussion                                                                                                                   28

5.2       Conclusion                                                                                                                  29

5.3       Recommendation                                                                                                        29

References                                                                                                                              30



 

LIST OF TABLES

TABLE          TITLE                                                                                                PAGES

4.1       Total Viable Count for Bacteria                                                                                 22

4.2       Microscopic and morphological characteristics fungal Isolated from stored files      23

4.4       Percentage occurrence of fungal isolated from stored Files                                       24

4.5       Total Viable Count Fungi                                                                                           25

4.6       Microscopy and Morphology Characteristics of Bacteria Isolated From Stored Files26

4.7       Zone of Inhibition of Isolated Organisms to Common Antibiotics                           27

Abstract

The mycological enumeration of stored files in the microbiology department was studied and carried out in the microbiology laboratory of Olabisi Onabanjo University. Bacteria were isolated by pour plate method on nutrient agar. Several tests including biochemical test like catalase test were carried out on the isolate from the stored files. The bacteria identified were Streptococci and Diplococci. The fungi identified were Aspergillus sp., Candida spp., Mould spp., Rhizopus spp., and Scopolariopsis spp. The percentage occurrence of the fungal isolates showed that, Aspergillus spp. were more predominant with the highest percentage with 32% and Candida spp. has the least percentage occurrence with 5%. Both Mould spp. and Rhizopus spp. had 23% each and Scopolariopsis spp. had 17%. These results underscore the importance of moderating human activities to limit biodeterioration of printed materials. Printed materials should be shielded away from direct sunlight or other UV-light sources that may encourage paper degradation.

Keywords: Stored Files, Bacteria, Fungi, biodeterioration. 

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