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ISOLATION AND CHARACTERISATION OF MALASSEZIA SPECIE FROM STUDENTS OF OLABISI ONABANJO UNIVERSITY

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Table of contents

 

CHAPTER ONE                                                                                                                     1 

1.1       Introduction                                                                                                                1 

1.2       General Objective                                                                                                       3 

1.3       Specific Objectives                                                                                                     3 

1.4       Significance of the Study                                                                                           3 

CHAPTER TWO: Literature Review                                                                                    4 

2.1       Malassezia                                                                                                                 

2.2       Pathophysiology on Human Skin                                                                               7 

2.3       Risk Factors for Malassezia Fungemia and Disseminated Disease                                    9 

2.4       Malassezia-Associated Skin Diseases                                                                        10 

2.4.1    Pityriasis versicolor                                                                                                    10 

2.4.2    Seborrheic dermatitis and dandruff                                                                            10 

2.4.3    Atopic dermatitis                                                                                                        11 

2.4.4    Malassezia folliculitis                                                                                                11 

2.4.5    Psoriasis                                                                                                                      12 

2.5       Detection of Malassezia Infections in Humans and Animals                                     13 

2.6       Susceptibility Testing and Treatment                                                                        15 

CHAPTER THREE: Materials and Methods                                                                         18 

3.1       Collection of Samples                                                                                                18 

3.2       Sterilization of Glassware                                                                                          18 

3.3       Microbiological Evaluation                                                                                        18 

3.4       Malassezia Species Identification                                                                              19 

3.4.2    Catalase Test                                                                                                               19 

CHAPTER FOUR: Results                                                                                                    20 

CHAPTER FIVE                                                                                                                    25 

5.1       Discussion                                                                                                                   25 

5.2       Conclusion and Recommendations                                                                            27 

References                                                                                                                              28 



 

LIST OF TABLES 

Table              Title                                                                                                    Pages 

4.1       Frequency and Percentage of Malassezia Species Isolated from Eczema 

and Dandruff                                                                                                               24 

Abstract

 

Malassezia species are known as the microflora of human skin that can induce superficial skin infections. The present study was carried out to determine the prevalence rate of Malassezia spp. among the students of the Olabisi Onabanjo University, Ago-Iwoye. Twenty (20) samples comprising of 10 samples of dandruff and 10 samples of eczema were collected randomly from twenty (20) students. A total twenty Malassezia spp. were identified based on their macroscopic, microscopic and physiological properties. The identified Malassezia spp. were Malassezia furfur, Malassezia restricta and Malassezia glubosa. The percentage occurrence of the isolated organisms showed 7 (35%) for Malassezia furfur, 7 (35%) for Malassezia glubosa and 6(30%) for Malassezia restricta. The presence of Malassezia spp. can cause severe disease, hence, the treatment of these infections should not be taken with levity. Medical practitioners should be consulted in cases associated with eczema and dandruff instead of self-medications. 

 

Keyword: Malassezia species, Microflora, Skin infection, Dandruff, Eczema. 

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