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ISOLATION AND CHARACTERIZATION OF SALMONELLA SPECIES IN PASTEURIZED MILK PRODUCTS MARKETED IN IJEBU-ODE, OGUN STATE

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Table of contents

CHAPTER ONE                                                                                        1

1.1      Introduction                                                                                     1

1.2      Aim of the Study                                                                              3

1.3      Specific Objectives                                                                          3

CHAPTER TWO: LITERATURE REVIEW                                               4

2.1      Salmonella spp.                                                                                4

2.2      Growth and survival characteristics                                                  5

2.3      Microbiological Quality of Pasteurized Milk                                     7

2.4      Factors Affecting Microbiological Quality of Pasteurized Milk   10

CHAPTER THREE: MATERIALS AND METHOD                                   18

3.1      Collection of Samples                                                                      18

3.2      Sterilization of Glass wares                                                              18

3.3      Preparation of Media                                                                        19

3.4      Isolation of Salmonella species                                                         19

3.5      Identification and Characterization of Salmonella spp.                       20

3.5.1   Microscopic Identification of Bacteria Isolates Gram Staining                   20

3.5.2   Indole Test                                                                                      21

3.5.3   Motility Test                                                                                    21

3.5.4   Oxidase Test                                                                                    22

3.5.5   Urease Test                                                                                      22

3.5.6   Citrate Test                                                                                      22

3.5.7   Sugar Fermentation Test                                                                   23

CHAPTER FOUR: RESULTS                                                                    24

CHAPTER FIVE                                                                                        28

5.1      Discussion                                                                                       28

5.2      Conclusion and Recommendations                                                   30

Reference                                                                                                  31

LIST OF TABLES

Table Title                                                                                                Page

3.1      Code of samples                                                                               18

4.1      Showing the total viable count of the milk samples                            25

4.3      Morphological and biochemical characteristics of isolates recovered

          from milk sample                                                                             26

4.3      Frequency and percentage of occurrence of isolated

          organisms from milk samples                                                           27.

Abstract

Milk and Milk products derived from dairy cow can harbour microorganisms and can serve as sources of foodborne pathogens. This study was carried out to determine the microbial loads and prevalence of Salmonella species in milk drinks marketed in Ijebu-ode, Ogun State. The microbial load was determined by serial dilution and spread plate techniques while the isolation and identification of Salmonella species was done using Salmonella Shigella agar and conventional biochemical methods. Four out of the nine products sampled had no microbial growth while the remaining five had total viable Salmonella count that ranged from 7.0 x 105 cfu/ml to 6.3 x 106 cfu/ml., with BBStar having the highest microbial load while Tropical drink has the lowest microbial load. Five species of Salmonella were isolated out of which three were Salmonella typhi and two were Salmonella paratyphi. The high microbial load and isolation of Salmonella species in these pasteurized milk drinks pose a health risk to consumers especially children.

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