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ISOLATION AND CHARACTERIZATION OF SALMONELLA SPECIES IN PASTEURIZED MILK PRODUCTS MARKETED IN IJEBU-ODE, OGUN STATE
Categories
Table of contents
CHAPTER ONE 1
1.1 Introduction 1
1.2 Aim of the Study 3
1.3 Specific Objectives 3
CHAPTER TWO: LITERATURE REVIEW 4
2.1 Salmonella spp. 4
2.2 Growth and survival characteristics 5
2.3 Microbiological Quality of Pasteurized Milk 7
2.4 Factors Affecting Microbiological Quality of Pasteurized Milk 10
CHAPTER THREE: MATERIALS AND METHOD 18
3.1 Collection of Samples 18
3.2 Sterilization of Glass wares 18
3.3 Preparation of Media 19
3.4 Isolation of Salmonella species 19
3.5 Identification and Characterization of Salmonella spp. 20
3.5.1 Microscopic Identification of Bacteria Isolates Gram Staining 20
3.5.2 Indole Test 21
3.5.3 Motility Test 21
3.5.4 Oxidase Test 22
3.5.5 Urease Test 22
3.5.6 Citrate Test 22
3.5.7 Sugar Fermentation Test 23
CHAPTER FOUR: RESULTS 24
CHAPTER FIVE 28
5.1 Discussion 28
5.2 Conclusion and Recommendations 30
Reference 31
LIST OF TABLES
Table Title Page
3.1 Code of samples 18
4.1 Showing the total viable count of the milk samples 25
4.3 Morphological and biochemical characteristics of isolates recovered
from milk sample 26
4.3 Frequency and percentage of occurrence of isolated
organisms from milk samples 27.
Abstract
Milk and Milk products derived from dairy cow can harbour microorganisms and can serve as sources of foodborne pathogens. This study was carried out to determine the microbial loads and prevalence of Salmonella species in milk drinks marketed in Ijebu-ode, Ogun State. The microbial load was determined by serial dilution and spread plate techniques while the isolation and identification of Salmonella species was done using Salmonella Shigella agar and conventional biochemical methods. Four out of the nine products sampled had no microbial growth while the remaining five had total viable Salmonella count that ranged from 7.0 x 105 cfu/ml to 6.3 x 106 cfu/ml., with BBStar having the highest microbial load while Tropical drink has the lowest microbial load. Five species of Salmonella were isolated out of which three were Salmonella typhi and two were Salmonella paratyphi. The high microbial load and isolation of Salmonella species in these pasteurized milk drinks pose a health risk to consumers especially children.
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